Cell culture medium ,DMEM/F-12

DMEM/F-12 medium was modified by mixing DMEM with Ham's F-12 medium at a ratio of 1:1. The modified DMEM/F-12 medium is richer in nutrients and contains more micronutrients, which is a widely used basal medium for supporting the growth of many different mammalian cells, as well as for the culture of mammalian cells at low serum levels and for clonal density culture. DMEM/F-12 has also been used as a base medium for the development of serum-free medium.